F plasmid derived origin used in most BACs. As a result, F’ factors can transmit bacterial genes from the F + cells to the F – cells. F- and I-complex replicons are typically associated with plasmids capable of conjugation, and the genes encoding components of the conjugal machinery typically take up 35 to 40 kb for both types of plasmids, although many of the F plasmids were missing at least 20% of the conjugation-associated genes … Fifteen of these genes are involved in F pilus synthesis and assembly [1, 2]. L S Chernin , M I Ovadis , and D M Goldfarb The F plasmid encodes approximately 40 genes within its transfer region. includes oriS, repE and the par genes ~4.6 kb; used in pBeloBAC11 and other BACs; at copy number 4-6 "complete" F origin. F plasmid: [ plaz´mid ] an extrachromosomal self-replicating structure found in bacterial cells that carries genes for a variety of functions not essential for cell growth. This process of gene transfer is called sexduction or F’ duction. They are designated F + simply because they have F plasmid. Interspecific Transfer of Plasmids: We know plasmid is an extrachromosomal DNA that can replicate independently. It is called F plasmid because it has F factor which is Fertility factor. traA encodes the precursor of the pilus subunit propilin; traQ and traX genes products are involved in the process of traA maturation. This fertility factor contains the genes require for the transfer or conjugation. Een plasmide is een cirkelvormige streng DNA die zich buiten het chromosomaal DNA bevindt van sommige eencellige organismen.Met dit DNA kan genetische informatie tussen bacteriën, ook tussen soorten, worden uitgewisseld.Dit is een vorm van horizontale genoverdracht.Via plasmiden worden vaak eigenschappen overgedragen die bijdragen aan de overleving van het organisme, bijvoorbeeld … Conjugative plasmids initiate gene transfer by altering the cell surface to allow contact between the plasmid-containing donor cell (F+ or male) and a plasmid lacking recipients (F- or female) Sex pilus originates from the donor and establishes conjugative bridge (temporary cytoplasmic bridge) that serves as the conduit for DNA transfer from donor to recipient bacterial cell F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K-12 HfrC. For example, F carrying the lac + gene will transmit it to an F – lac – cell; as a result, the latter will be converted into a lac + cell.

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